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Coding

Part:BBa_K1554001:Design

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-26)


AtrΔ11


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Nicotiana benthamiana was performed before ordering sequence by gBlock. Original sequence accession number: [http://www.ncbi.nlm.nih.gov/nuccore/JX964774 JX964774].

It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (AATG and GCTT). This part was sent to the Registry of Parts using RFP coding device - Golden Gate Module Flipper.

Source

Ordered by gBlock.

References

[1] Choi MY, Han KS, Boo KS, Jurenka RA (2002) Pheromone biosynthetic pathways in the moths Helicoverpa zea and Helicoverpa assulta. Insect Biochem Mol Biol. 32, 1353-9.

[2] Wang HL, Zhao CH, Wang CZ (2005) Comparative study of sex pheromone composition and biosynthesis in Helicoverpa armigera, H. assulta and their hybrid. Insect Biochem Mol Biol. 35, 575-83.

[3] Fu X, Fukuzawa M, Tabata J, Tatsuki S, Ishikawa Y (2005) Sex pheromone biosynthesis in Ostrinia zaguliaevi, a congener of the European corn borer moth O. nubilalis. Insect Biochem Mol Biol. 35, 621-6.

[4] Matsumoto S (2010) Molecular mechanisms underlying sex pheromone production in moths. Biosci Biotechnol Biochem. 74, 223-31.

[5] Gu SH, Wu KM, Guo YY, Pickett JA, Field LM, Zhou JJ et al (2013) Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport. BMC Genomics. 14, 636.

[6] Hagström Å, Wang HL, Liénard MA, Lassance JM, Johansson T, Löfstedt C (2013) A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory. Microb Cell Fact. 12, 125.

[7] Ding BJ, Hofvander P, Wang HL, Durrett TP, Stymne S, Löfstedt C (2014) A plant factory for moth pheromone production. Nat Commun. 5, 3353. [8] Liu W, Jiao H, O'Connor M, Roelofs WL (2002) Moth desaturase characterized that produces both Z and E isomers of delta 11-tetradecenoic acids. Insect Biochem Mol Biol. 32, 1489-95.

[9] Rodríguez S, Hao G, Liu W, Piña B, Rooney AP, Camps F et al (2004) Expression and evolution of delta9 and delta11 desaturase genes in the moth Spodoptera littoralis. Insect Biochem Mol Biol. 34, 1315-28.